Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation (2024)

Marchioli, G., van Gurp, L., van Krieken, P. P., Stamatialis, D., Engelse, M., van Blitterswijk, C. A., Karperien, M. B. J., de Koning, E., Alblas, J., Moroni, L. (2015). Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation. Biofabrication, 7(2), Article 025009. https://doi.org/10.1088/1758-5090/7/2/025009

Marchioli, G. ; van Gurp, L. ; van Krieken, P. P. et al. / Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation. In: Biofabrication. 2015 ; Vol. 7, No. 2.

@article{cf2696f3d7ce410bac4bb078bf36eb8b,

title = "Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation",

abstract = "In clinical islet transplantation, allogeneic islets of Langerhans are transplanted into the portal vein of patients with type 1 diabetes, enabling the restoration of normoglycemia. After intra-hepatic transplantation several factors are involved in the decay in islet mass and function mainly caused by an immediate blood mediated inflammatory response, lack of vascularization, and allo- and autoimmunity. Bioengineered scaffolds can potentially provide an alternative extra-hepatic transplantation site for islets by improving nutrient diffusion and blood supply to the scaffold. This would ultimately result in enhanced islet viability and functionality compared to conventional intra portal transplantation. In this regard, the biomaterial choice, the three-dimensional (3D) shape and scaffold porosity are key parameters for an optimal construct design and, ultimately, transplantation outcome. We used 3D bioplotting for the fabrication of a 3D alginate-based porous scaffold as an extra-hepatic islet delivery system. In 3D-plotted alginate scaffolds the surface to volume ratio, and thus oxygen and nutrient transport, is increased compared to conventional bulk hydrogels. Several alginate mixtures have been tested for INS1E ?-cell viability. Alginate/gelatin mixtures resulted in high plotting performances, and satisfactory handling properties. INS1E ?-cells, human and mouse islets were successfully embedded in 3D-plotted constructs without affecting their morphology and viability, while preventing their aggregation. 3D plotted scaffolds could help in creating an alternative extra-hepatic transplantation site. In contrast to microcapsule embedding, in 3D plotted scaffold islets are confined in one location and blood vessels can grow into the pores of the construct, in closer contact to the embedded tissue. Once revascularization has occurred, the functionality is fully restored upon degradation of the scaffold.",

keywords = "type 1 diabetes, beta cells, bioplotting, tissue engineering, islets of Langerhans",

author = "G. Marchioli and {van Gurp}, L. and {van Krieken}, {P. P.} and D. Stamatialis and M. Engelse and {van Blitterswijk}, {C. A.} and Karperien, {M. B. J.} and {de Koning}, E. and J. Alblas and L. Moroni and {van Apeldoorn}, {A. A.}",

year = "2015",

month = jun,

doi = "10.1088/1758-5090/7/2/025009",

language = "English",

volume = "7",

journal = "Biofabrication",

issn = "1758-5082",

publisher = "IOP Publishing Ltd.",

number = "2",

}

Marchioli, G, van Gurp, L, van Krieken, PP, Stamatialis, D, Engelse, M, van Blitterswijk, CA, Karperien, MBJ, de Koning, E, Alblas, J, Moroni, L 2015, 'Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation', Biofabrication, vol. 7, no. 2, 025009. https://doi.org/10.1088/1758-5090/7/2/025009

Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation. / Marchioli, G.; van Gurp, L.; van Krieken, P. P. et al.
In: Biofabrication, Vol. 7, No. 2, 025009, 06.2015.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation

AU - Marchioli, G.

AU - van Gurp, L.

AU - van Krieken, P. P.

AU - Stamatialis, D.

AU - Engelse, M.

AU - van Blitterswijk, C. A.

AU - Karperien, M. B. J.

AU - de Koning, E.

AU - Alblas, J.

AU - Moroni, L.

AU - van Apeldoorn, A. A.

PY - 2015/6

Y1 - 2015/6

N2 - In clinical islet transplantation, allogeneic islets of Langerhans are transplanted into the portal vein of patients with type 1 diabetes, enabling the restoration of normoglycemia. After intra-hepatic transplantation several factors are involved in the decay in islet mass and function mainly caused by an immediate blood mediated inflammatory response, lack of vascularization, and allo- and autoimmunity. Bioengineered scaffolds can potentially provide an alternative extra-hepatic transplantation site for islets by improving nutrient diffusion and blood supply to the scaffold. This would ultimately result in enhanced islet viability and functionality compared to conventional intra portal transplantation. In this regard, the biomaterial choice, the three-dimensional (3D) shape and scaffold porosity are key parameters for an optimal construct design and, ultimately, transplantation outcome. We used 3D bioplotting for the fabrication of a 3D alginate-based porous scaffold as an extra-hepatic islet delivery system. In 3D-plotted alginate scaffolds the surface to volume ratio, and thus oxygen and nutrient transport, is increased compared to conventional bulk hydrogels. Several alginate mixtures have been tested for INS1E ?-cell viability. Alginate/gelatin mixtures resulted in high plotting performances, and satisfactory handling properties. INS1E ?-cells, human and mouse islets were successfully embedded in 3D-plotted constructs without affecting their morphology and viability, while preventing their aggregation. 3D plotted scaffolds could help in creating an alternative extra-hepatic transplantation site. In contrast to microcapsule embedding, in 3D plotted scaffold islets are confined in one location and blood vessels can grow into the pores of the construct, in closer contact to the embedded tissue. Once revascularization has occurred, the functionality is fully restored upon degradation of the scaffold.

AB - In clinical islet transplantation, allogeneic islets of Langerhans are transplanted into the portal vein of patients with type 1 diabetes, enabling the restoration of normoglycemia. After intra-hepatic transplantation several factors are involved in the decay in islet mass and function mainly caused by an immediate blood mediated inflammatory response, lack of vascularization, and allo- and autoimmunity. Bioengineered scaffolds can potentially provide an alternative extra-hepatic transplantation site for islets by improving nutrient diffusion and blood supply to the scaffold. This would ultimately result in enhanced islet viability and functionality compared to conventional intra portal transplantation. In this regard, the biomaterial choice, the three-dimensional (3D) shape and scaffold porosity are key parameters for an optimal construct design and, ultimately, transplantation outcome. We used 3D bioplotting for the fabrication of a 3D alginate-based porous scaffold as an extra-hepatic islet delivery system. In 3D-plotted alginate scaffolds the surface to volume ratio, and thus oxygen and nutrient transport, is increased compared to conventional bulk hydrogels. Several alginate mixtures have been tested for INS1E ?-cell viability. Alginate/gelatin mixtures resulted in high plotting performances, and satisfactory handling properties. INS1E ?-cells, human and mouse islets were successfully embedded in 3D-plotted constructs without affecting their morphology and viability, while preventing their aggregation. 3D plotted scaffolds could help in creating an alternative extra-hepatic transplantation site. In contrast to microcapsule embedding, in 3D plotted scaffold islets are confined in one location and blood vessels can grow into the pores of the construct, in closer contact to the embedded tissue. Once revascularization has occurred, the functionality is fully restored upon degradation of the scaffold.

KW - type 1 diabetes

KW - beta cells

KW - bioplotting

KW - tissue engineering

KW - islets of Langerhans

U2 - 10.1088/1758-5090/7/2/025009

DO - 10.1088/1758-5090/7/2/025009

M3 - Article

C2 - 26019140

SN - 1758-5082

VL - 7

JO - Biofabrication

JF - Biofabrication

IS - 2

M1 - 025009

ER -

Marchioli G, van Gurp L, van Krieken PP, Stamatialis D, Engelse M, van Blitterswijk CA et al. Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation. Biofabrication. 2015 Jun;7(2):025009. doi: 10.1088/1758-5090/7/2/025009